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KMID : 0603820060120040451
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Journal of Experimental & Biomedical Science
2006 Volume.12 No. 4 p.451 ~ p.455
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Effect of Allopurinol on Methylmercuric Chloride-Induced Cytotoxicity in C_6 Cultured Glioma Cells
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Oh Yong-Leol
Son Byoung-Kwan
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Abstract
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It is demonstrated that inorganic mercury has cytotoxic effect on glial cells. Recently, oxygen radicals is involved in methylmercuric chloride (MMC)-induced cytotoxicity. But, the toxic mechanism of MMC is left unknown. The purpose of this study was to examine the cytotoxicity of MMC on C_6 glioma cells. The cytotoxicy was measured by cell viability using XTT assay in C_6 glioma cells. Colorimetric assay is regarded as a very sensitive screening method for the determination of the cell viability on various agents. In this study, MMC decreased cell viability according to the dose- and time dependent manners after C_6 glioma cells were grown with various concentrations of MMC for 48 hours. In the protective effect of allopurinol on MMC-induced cytotoxicity, allopurinol was effective in the prevention of MMC-induced cytotoxicity in these cultures. These results suggest that MMC has highly cytotoxic effect on C_6 glioma cells by the decrease of cell viavility, and free radical scavenger such as allopurinol was effective on organic mercury-induced cytotoxicity in these cultures.
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KEYWORD
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Methylmercuric chloride, Cytotoxicty, Allopurinol
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